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However, false negatives will occur when the virus content is low.
This chapter reviews the current knowledge on reduction of the human pathogenic virus content in drinking water through treatment processes.
When the virus content is relatively low, the performance of real-time PCR is even better than that of traditional PCR.
Virus content was determined by standard hemagglutination (HA) assay [17].
Data from a semi-quantitative analysis of the virus content in blood samples by real-time RT-PCR were compared with virus isolation data (Table 1).
On the one hand, RT-PCR in general is a very robust and sensitive technique which enables us to sequence whole genomes from samples with a broad range of virus content.
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The best virus capture factor f (ratio of upstream to downstream virus contents) was obtained when using 2 functionalized filters (f = 2.9 × 103).
The blood virus contents in the 1 and 2 groups were significantly lower than that of the VC group.
At the 8th hour after DHAV injection the virus contents of the 1 and 2 groups were lower than that of the VC group with significant difference.
The 1 and 2 groups' blood virus contents were lower than that of the VC group and this is especially significant for the 2 group.
To monitor the blood virus contents in virusemia phases (the 4thh and the 8thh) and stable (the 54thh) phases of the disease, blood samples randomly taken from 5 feathers per group at the 4thh, the 8thh and the 54thh after challenged with virus were collected, treated with heparin anticoagulation and used for testing its virus nucleic acid contents.
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