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Exon reads were assembled together to create virtual transcript for each primate using the Sequencher® program (version 4.5, www.genecodes.com) and visually checked for accuracy.
It may also erroneously assign virtual transcript to a pseudogene which is not expressed.
Microarray probes were designed, one per gene, to the region closest to the 3' untranslated region (UTR) of each rhesus virtual transcript.
The large amount of DNA sequence data available for several species has been used for in silico predictions of virtual transcript profiles.
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The remaining species added up to 11.7% of virtual transcripts analysed.
We analysed 560,360 virtual transcripts with the SSRLocator software (Table 1).
Gene Indices developed by TIGR were created by assembling ESTs into virtual transcripts, which were named tentative consensus (TC) records.
Gene Indices developed by TIGR[ 28] were created by assembling ESTs into virtual transcripts, named TC records.
The frequency of SSR per EST database was higher (4.66%) in Selaginella spp virtual transcripts (Table 2).
BlastX searches with the virtual transcripts of the isoforms within this set retrieved an additional 467 Arabidopsis, 1,219 rice and 38 maize annotated proteins.
EST clustering yielded a database that covers a large proportion of the transcriptome, approximately 8% of the virtual transcripts contain full-length CDS.
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CEO of Professional Science Editing for Scientists @ prosciediting.com