The performance was evaluated by two expert pathologists interacting with a set of 20 virtual slides.
We aim at segmenting very large images of histopathology virtual slides with an heterogeneous and complex content.
The method is applied to the segmentation of various types of ovarian carcinoma stroma, on very large virtual slides.
It uses a novel method for slice-to-slice image registration using automatic registration algorithms custom designed for both virtual slides and histopathological images.
However, the slight time increase using virtual slides is less than that using dynamic telepathology and traditional consultation, and is acceptable to the pathologists.
Whole slide images (WSIs), also known as virtual slides, can support electronic distribution of immunohistochemistry (IHC) stains to pathologists that rely on remote sites for these services.
We describe a novel system for 3D histological reconstruction, integrating whole-slide imaging (virtual slides), image serving, registration, and visualization into one user-friendly package.
The unique design of the LVM, enabling real-time rendering of virtual slides while providing users with a quick and intuitive way to navigate within and between slides, makes use of digital pathology in routine practice a realistic possibility.
This system has been applied to >300 separate 3D volumes from eight different tissue types, using a total of 5500 virtual slides comprising 1.45 TB of primary image data.
Although the preparation and subsequent digitization of zebrafish larval histology specimens can be conducted in parallel, the scoring and annotation of the resulting virtual slides is largely manual and therefore rate limiting, which motivates the development of systems for automated characterization of histology images.
Click here for a video tutorial on using the Aperio ImageScope client to view virtual slides (NOTE: the ImageScope client is a WINDOWS-only application).
