Since these boundary conditions can develop progressive moments, the ideal simple supported bi-articulated configuration of the actuator is no longer valid, and virtual clamped configuration appears with friction moments that substantially modify the buckling analysis.
TEVC recording used HS2A head stages and a VG-2Ax100 virtual bath clamp connected to a Gene Clamp 500 amplifier interfaced through a Digidata 1440A digitizer acquired using pClamp10 software (all Axon Instruments).
To add virtual conductances, the dynamic clamp technique was applied using Signal 5 software (Cambridge Electronic Design).
You may not be able to remove the triggers, but you can reprogram the thing itself, a kind of virtual bottle that automatically clamps shut when you've had too much to drink or a casino that turns off its lights as you move into dangerous territory.
(C ) Prediction 2 was tested by adding virtual sodium conductance via dynamic clamp.
Table 1 Proposed switching combination and virtual DC link voltage Sextant Clamped switch Modulated switches Virtual DC link voltage 1 R Y' W' vRY vRW 2 W' R Y vRW vYW 3 Y W' R vYW vYR 4 Y W vYRvWR vWR 5 W R' Y' vWR vWY 6 Y' W R vWY vRY.
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Nonetheless, based on an unsuspected difficulty with pharmacologically converting muscle afferents (despite the ease of converting them with virtual sodium current), we conducted voltage clamp experiments to test whether muscle afferents express less dendrotoxin-sensitive potassium current.
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When human sperm monovalent CatSper currents were measured by whole-cell patch-clamping, the I V curve showed a virtual absence of inward current, as described by Lishko et al. [ 11] using the same recording conditions.
