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This study aimed to isolate viral particles of BPV from skin papillomas, using a novel viral isolation method.
Viral isolation is considered to be the gold standard for detection of pathogen infection.
Moreover, viral isolation, endpoint dilution assays, and plaque assays are only able to identify infectious viral particles.
Laboratory diagnosis of influenza has previously relied on viral isolation in culture.
Patients were followed up by pp65 antigenemia and by viral isolation from blood or/and urine samples.
Consequently, acute infection has to be demonstrated by direct detection of the virus by viral isolation or genomic amplification.
Viral isolation is difficult and has low sensitivity, and consequently RT-PCR assays are currently used for detection.
Initially, laboratory diagnosis was done by immunofluorescence (IF) assay on clinical samples, followed by viral isolation in Madin Darby canine kidney (MDCK) cells.
Viral isolation from CSF has limited clinical value due to low virus recovery; the cause for which has not been demonstrated.
Cytomegalovirus infection in neonates was determined by means of urinary viral isolation during the first week after birth or the histologic examination of tissue from aborted fetuses.
Background: The viral isolation technique (VIT) is largely used as a gold standard for the detection of influenza A and B viruses in respiratory samples.
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CEO of Professional Science Editing for Scientists @ prosciediting.com