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Plant-related experiments, such as agro-infiltration and viral inoculation were performed in a greenhouse (16 h light/8 h darkness, 20°C to 25°C).
All experiments were subsequently repeated with compounds added to wells just after viral inoculation.
Mortality occurred within 10 days of the viral inoculation, and observation for 21 days after infection showed no subsequent mortality.
Acute HCV infection is difficult to study due to the lack of clinical disease around the time of viral inoculation.
Prior to viral inoculation, mice were anesthetized by i.p. administration of xylazine (7 mg/kg) and ketamine (100 mg/kg).
The activity of T cells, is keenly important following cutaneous viral inoculation, and they are key in bridging the innate and adaptive immune response [62].
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After viral inoculations, we measured ABRs in adult mice at time points given in the Results.
We have repeated viral inoculations using solutions independently made from three batches.
One week before the VEEV inoculations, all horses were treated with insecticide to eliminate external parasites, and their temperature and blood counts were recorded 3 days before the viral inoculations.
Six days post-viral inoculation mice were euthanized for assessment of viral load in the lungs.
Levels of the cytokines TNF-α, IFN-γ, IL-6, IL-12 and IL-1β were also significantly reduced by PT treatment on day 2 post-viral inoculation.
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