To see if you can afford to unlock a skill, check your current skill point balance displayed on the top-right corner of the screen, in big blue-violet numbers.
QTL for root to shoot ratio (R/S, violet), root number (RN, green) maximum root length (MRL, red), and root thickness (THK, blue).
Colonies were fixed with methanol and stained with 0.5% crystal violet; the number of colonies containing at least 50 cells was determined and surviving fraction was calculated.
After 14 days, cells were fixed and stained with crystal violet and number of colonies greater than 5 mm diameter counted.
Eight hours post-plating, cells were fixed with 10% NBF, stained with 0.1% crystal violet and number of motile cells quantitated as previously described [ 15].
Nine days after treatment, cells were stained with crystal violet, the number of colonies containing at least 50 cells was counted [ 15] and the effective half inhibitory dose (IC50) of the toxin was calculated on the basis of linear regression equation.
If anyone is thinking of getting this done, perhaps they could take inspiration from Palma Violets' closing number.
Separated osteogenic and adipogenic cultures were also stained with crystal violet, and the number of total cell colonies were counted.
Sections were counterstained with cresyl violet and the number of dopaminergic neurons counted using the optical fractionator technique (StereoInvestigator, MBF Bioscience, Williston, VT).
The membranes were wiped to remove the matrigel and fixed with 4% paraformaldehyde, stained with crystal violet and the number of cells that had invaded through to the other side of the membrane were counted.
