Disability Rights UK will continue to campaign vigorously to fix a broken policy, and be a strong voice for disabled people.
Since taking office, Sigurdardottir, 66, has been working vigorously to fix the broken banking system.
Cell pellets were resuspended vigorously and fixed with 70% ethanol and kept at −20°C for 2 hours.
JEG-3 cells growing in parallel were trypsinized, passaged through a 40 µm nylon sieve, plated onto the ECM deposited by the transfected JEG-3 cell lines and cultured for 30 min. Cells were washed vigorously in 1×PBS, fixed in 4% paraformaldehyde and stained with eosin.
In and around Porterville, Dreamers and their backers angrily question why McCarthy and fellow congressmen from California's Central Valley aren't vigorously pushing for a legislative fix — pronto — because so many Dreamers' lives hang in the balance. .
Recent developments in fixed point theory reflect that the iterative construction of fixed points is vigorously proposed and analyzed for various classes of maps in different spaces.
After 30 min at 37°C and 5% CO2, coverslips were washed vigorously with phosphate buffered saline (PBS), fixed in 1.8% formaldehyde for 5 min, and then submerged in PBS until counted.
For whole-mount staining, human synovial tissues from RA patients were washed vigorously in ice-cold PBS, then fixed and permeabilized with a BD Cytofix/Cytoperm Kit (BD Biosciences, San Jose, CA, USA).
Our system is broken – so don't fix it, says Cameron now, campaigning vigorously against electoral reform, stuffing an unreformed House of Lords with party placelings, and insisting only on a redrawing of constituency boundaries that benefits his party.
Cells were fixed in 2 mL 70% ethanol (stored at −20°C), vigorously vortexed and incubated at 4°C for 4 hr.
