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However, in most cases only initial verification of detected biomarkers was conducted, but appropriate vigorous validation in a sufficiently large population is still outstanding.
Subjecting them through a vigorous validation process using high-quality, well-annotated clinical specimens is necessary.
Without vigorous validation, it is not at all clear how reliable putative gene classifiers are for chemical exposures.
Second, our Satisfaction with Mental Health Services Scale was self-constructed, and further and more vigorous validation of the scale is needed.
Upon vigorous validation and improvement, the current model can potentially be applied to integrate preclinical and clinical data, and ultimately aid the risk assessment of immunogenicity for therapeutic proteins.
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Reasons for this lack of adoption include doubts about the accuracy and applicability of models to contemporary practice, lack of vigorous model validation, the inability to easily perform model calculations and uncertainty over how such models might be used in clinical practice.
What will validation validate?
Many such classifiers discovered thus far lack vigorous statistical and experimental validations.
And many proposed ecotoxicological gene classifiers are simply differentially expressed genes lacking vigorous statistical and experimental validations.
The SCDR participates in international multicentre studies and has a level of ascertainment approaching 100% through efficient entry of incident cases, stringent validation procedures and vigorous data collection.
We hopefully anticipate that dissemination and external validation of our findings will generate vigorous discussion and continuous investigation in search of proper prognostic markers.
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