After seven days of cultivation, cells were harvested by vigorous pipetting and transferred into 5 mL tubes (Becton Dickinson, San Jose, CA, USA).
HMDMs were removed from wells by vigorous pipetting and transferred to 5 mL tubes, centrifuged at 1000 rpm for 10 min, and fixed in 1% paraformaldehyde (150 μL).
5 mL of Dimetrol® suspension was accurately transferred (after vigorous shaking) into a 100 mL measuring flask, sonicated in 50 mL methanol for 10 min and filtered into another 100 mL volumetric flask.
The New York Voices have a smooth blend and a comfortable mastery of pop-jazz harmony not unlike the far more vigorous, swinging and adventurous Manhattan Transfer.
The U-turn came after MPs and hundreds of thousands of members of the public joined the vigorous protests against moves to transfer 637,000 acres of woodlands into private ownership.
These shoots showed continued and vigorous growth and development upon transfer onto fresh hygromycin medium.
But welfare states are meant to transfer resources from the vigorous to the fragile.
Dr. Irvine's specialty was heat and mass transfer and he was a vigorous advocate of international cooperation in his field.
Therefore, a vigorous mixing of fuel that enhances heat transfer is required.
Remarkably, adoptively transferred CD8+ T cells underwent vigorous proliferation not only in lymph nodes and peripheral blood, but also in the ascites and tumor compartments.
