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Under vigorous stirring, solutions of mercaptoethyl ether (4.5 mL, 36 mmol) and dibromoethane (3.2 mL, 37 mmol) in dimethylformamide (40 mL, 40 mL) were added dropwise at a rate of 1.2 mL per hour.
After vigorous mixing the solution was centrifuged and clear evidence of phase separation was observed.
After vigorous mixing this solution was allowed to sit overnight in the dark.
After vigorous vortexing, the solution was then centrifuged at 20,000× g at 4°C for 10 min and then the supernatant was dried under vacuum.
Na-deoxycholate was added to a final concentration of 1% (w/vol) and after vigorous shaking the solution was incubated at 8°C for 6 h.
After 10 min, under vigorous magnetic stirring, solution of 0.08 M cerium IV ammonium nitrate (CAN) in 0.2 M nitric acid was added to obtain a 5 mL solution.
After a minute or so of vigorous stirring, the solution should be completely dissolved.
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In this process, cationic dyes were added into pre-formed PVB solutions and dissolved in the solutions by vigorous stirring and then cationic dye colored PVB solutions can be e-spun into colorful nanofibers and collected as colorful nanofibrous membranes (NFMs).
