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The group show at Artists Space is a small but vigorous sampling of what is emerging from the project.
It is important to consider that collection of the sample should be done with the same strength and skill to obtain a single layer of epithelia; in other words, sampling method should be kept constant since, repeated and vigorous sampling may lead to collection of cells from the less differentiated basal layer.
It is essential to stress that swab samples should be obtained by vigorous sampling to acquire sufficient biologic material for testing, and development of a quality-control PCR target (housekeeping gene target) would be beneficial for sample integrity assessment, which is a limitation of this study.
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After vigorous stirring, the sample froze instantly.
After vigorous mixing, the samples were centrifuged at 5000 r.p.m. for 5 min at 4 °C.
The tissues were homogenized with vigorous vortexing until sample was completely resuspended.
Following a brief but vigorous vortex the samples were centrifuged at 14 000 r.p.m. for 10 min at 4°C.
Cell pellets were resuspended by vigorous vortexing in sample buffer containing 3% SDS, 100 m M DTT, 60 m M Tris (pH 6.8), 0.01% bromophenol blue and 10% glycerol.
Therefore, both PA6/clay nanocomposite samples did not produce sufficient amounts of protective floccules to cover the entire sample surface and vigorous bubbling was observed over the sample surface which was not covered by the protective floccules.
After the improvement, the oxidation reduction property is above +100 mV and the oxidation activities in samples are vigorous.
Vigorous stirring ensured the sample's homogeneity and that the X-ray beam (1 mm diameter) passed through a representative sample of the ampoule's contents.
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