Although the thousands of vigorous medium-sized German concerns are less visible than the country's titans (partly because they choose to be), they are the most enterprising part of the economy.
The long term culture showed that EPT1 cells not only grew faster, but also looked more vigorous in medium containing 5% FCS than in the standard EP156T medium, so the concentration of FCS in the medium for EPT1 cells was adjusted from 1% to 5%.
The objective of this study was to explore the association between time spent on medium, vigorous physical activity (MVPA) and concurrent gains in BMI, fat mass (FM) and fat-free mass (FFM), alternately, in a cohort of Mexican children followed from kindergarten (baseline) to 2nd grade elementary school (endline).
T. thermophilus HB27 (DSM 7039) and its derivative strains were grown at 60°C or 70°C with vigorous shaking in rich medium (TB) or nutritionally defined medium (SH).
Protocells, which are more vigorous in a fluid medium, could help buildings better withstand wet conditions.
S. cerevisiae W303-1A cells were grown at 30°C for 16 h with vigorous shaking in YPD medium containing 1% (w/v) yeast extract, 2% (w/v) peptone, and 2% (w/v) D-glucose.
For in vitro assays, the 500 µM glycolipid stocks in DMSO were diluted directly into prewarmed (37°C) culture medium with vigorous mixing and 10 minutes of sonication.
E. coli strains were grown at 37°C under vigorous agitation in LB medium [47] with ampicillin (100 µg.ml-1), erythromycin (150 µg.ml-1) or tetracycline (10 µg.ml-1) when required.
Melanized microsclerotial formation was observed for both wild type V592 and vdgarp1 mutant spores in infertile nutrient agar medium, with vigorous mycelial growth in the vdgarp1 mutant but not in V592 (Figure 6D and 6E).
The headspace gas was mixed with the medium by vigorous agitation before starting the culture.
