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After incubation for 1 h, the monolayers were washed three times with HBSS and lysed by vigorous aspiration with 1 ml phosphate buffered saline (PBS; pH 7.4) containing 0.1% (wt/vol) sodium deoxycholate.
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As celebrity and mass-market fragrances peddle the usual plots of desire and aspiration (often in steamy commercials with vigorous hair-tossing), smaller players have found success with desexualized, sometimes unusual back stories.
This second significance level was chosen to conform with the argument that for the detection of intended changes in complex social systems lower aspirations should be acceptable and less vigorous standards should be targeted [ 17].
As well as strong aspiration there are other causes of hemolysis, including vigorous shaking of the tube, transportation by pneumatic post within the clinic, centrifugation at too high speed, and inadequate environmental temperature.
The aspiration was for the participants to engage in moderate or vigorous activity for 150 minutes a week in bouts of at least 10 minutes, but if that seemed unrealistic then the facilitator encouraged any increase in physical activity, whatever the intensity.
NaturallyCurly styles itself as a rialto for people with curly hair, and the vigorous participation of advertisers on the site suggests that it has carried off this aspiration.
Low aspiration begets low aspiration.
Vigorous attempted procreation.
Vigorous debate has ensued.
Redistribution is vigorous.
Vigorous pelvic wallop?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com