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For quantification of β-cell area, sections were viewed at a magnification of 10×.
Slides were viewed at a magnification of 400 on a Leitz fluorescence microscope within 24 hours by two independent observers.
Cells were viewed at a magnification of × 63 with an oil-immersion objective and representative cells photographed.
Tissue sections were viewed at a magnification of 400× and non-overlapping fields were evaluated without knowledge of animal or treatment identification.
Cells were rinsed with PBS, mounted on slides with GelMount, and viewed at a magnification of 1000× on a Zeiss Axioskop microscope, with images collected by a Spot Insight QE digital camera.
The stained sections were viewed at a magnification of 200 times and photographed with eclipse 50i optical microscope imaging system (Nikon, Co. Ltd ,Toyko, Japan), and the images were analyzed by Image-Pro-plus sofeware (Media CyBaltimore, BaltiMDre, MD).
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Stained sections were viewed at a 10× magnification and the middle portion of each section was photographed.
Individual comets were viewed at a final magnification of × 400 using a fluorescence microscope (Eclipse E600, Nikon, Tokyo, Japan) equipped with 590 nm long-pass emission filter.
Microscopic images of alveoli were viewed at a final magnification of 130× with a color video camera (model CCD SSC-S20; SONY, Tokyo, Japan) and recorded on Pinnacle Studio Plus software (Pagasus Imaging Corporation Tampa, FL).
Microscopic images of alveoli were viewed at a final magnification of 130× with a color video camera (model CCD SSC-S20; Sony, Tokyo, Japan) and recorded on Pinnacle Studio Plus software (Pagasus Imaging Corporation Tampa, FL) Each field measured 1.22 × 106 μm2 and was filmed throughout five complete tidal ventilations for subsequent analysis of alveolar mechanics.
Stained specimens were viewed at an objective magnification of ×100 and ×200 by two investigators (HJH and CH).
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