A light source, set at right angles to a viewing microscope, illuminates the oil droplets and makes them appear as bright stars while they fall.
As each alpha particle struck the fluorescent screen, it would produce a burst of light called a scintillation, which was visible through a viewing microscope attached to the back of the screen.
In few cases with discrepancy, between the two observers, the immunostained slides were reviewed in a double viewing microscope so that the discrepancy was settled.
The variation of the droplet volumes is an important subject, but using a side-viewing microscope to profile a large number of small immersed droplets is labor-intensive and impractical.
When the scores differed between observers, they were resolved by discussion at a dual-view microscope.
We multiplied this value by the constant 0.78125 μm, representing the height and width of each pixel in the field, and divided by the constant 0.09 mm, the area examined by the zeroed 300-μm field-of-view microscope lens to produce a CNFL value in millimeters per square millimeter.
ERCC1 immunohistochemical staining was assessed by two blinded investigators (J Sung and GY Kwon) who reviewed the cases simultaneously at a multihead viewing microscope.
In the few cases with discrepancy between the two observers, the immunostained slides were reviewed in a double viewing microscope so that the discrepancy was settled.
In few cases with discrepancy, between the two observers, greater than 10%, the immunostained slides were reviewed in a double viewing microscope so that the discrepancy was settled.
To allow enough fluorescence light to be collected by the low-NA side-viewing microscope, many traces were averaged with a CCD exposure time of 8 s.
Measurement of changes in fluorescence, as an indicator of [Ca2+] i, was observed using a PerkinElmer Ultra VIEW confocal microscope.
