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In agreement with the results of transcriptome-wide analysis of mammalian mRNA folding, we found that secondary structures are frequently formed in PK transcripts between UTRs and CDSs in the vicinity of the start and stop codons (Figure S4).
Transcript folding in the vicinity of the start codon favors formation of characteristic local structures where the start codon is positioned at the end of a hairpin in a relaxed loop [30].
For example, the in vivo occupancy profile (shown in blue) has a very pronounced peak in the vicinity of the start codon of YAL053W (marked with an arrow in Figure 2), which presumably corresponds to the +1 nucleosome immediately downstream of the transcriptional start site of this gene.
To characterize further the diversity of the finTRIMs, we performed a 3'RACE PCR on VHSV-induced leukocyte cDNA using a universal primer specific for trout finTRIM localized in the highly conserved region in the vicinity of the start codon.
The nucleotide sequences in the vicinity of the start codon (from −100 to +24 and −30 to +24 relatives to ATG) were also obtained from RSAtools (retrieve sequence section, default parameters).
The methylation status of nine CpG sites near the transcriptional start site and histone H3-K9 modinicathen in the vicinity of the start site were both related to MUC1 gene expression.
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Since now the guess pulse already has a relatively high fidelity, both the (J_{T}^{mathrm {sm}}) and (J_{T}^{mathrm {geo}}) functionals may be used interchangeably, as we are only searching in a very small vicinity of the starting point.
Besides understanding the types of protein families that are in the vicinity of the starting sequence, the purpose of performing fan-like search is also to determine if the search space of the starting sequence for its orthologous sequences is well sampled.
In addition, Sp1 (threshold 91), NF-Y (threshold 90), CREB (threshold 100), and Myb (threshold 88) consensus sites were identified at the vicinity of the transcriptional start site of the CDT2 gene (Fig. 1A).
We also found that CTCF depletion was associated with higher levels of an antisense transcript, and heterochromatin formation involving the critical +1 nucleosome in the vicinity of the transcription start site (TSS) of the FXN gene.
Also it is possible that such a change in the conformation of the RNA might recruit certain other trans acting factors near the vicinity of the translation start site that would facilitate the recruitment of the ribosomes to the RNA and thus facilitating translation initiation.
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