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The vials were removed from storage ~20 at a time and allowed to melt at room temperature for one hour.
At fixed intervals (5 min, for 1 h), the two vials were removed, and lycopene content was determined.
After one week the vials were removed and replaced with new vials for a second week.
To determine where clonogenic and differentiation potential is lost during lyophilization, vials were removed at the end of primary drying (−35°C) and during secondary drying (at 5°, 10°, 15°, and 20°C) from the freeze-drying chamber into an antechamber using a retractable arm.
A representative plot of the temperature profile during the freeze drying cycle shows when vials were removed during lyophilization without interrupting the cycle to assess the effect of each step on the differentiation and clonogenic potential of the cells (Figure 3).
After one hour, females from the vials were removed using light CO2 and were discarded.
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After hydrolysis, the vials are removed from the heat and left to stand until cool.
Vials are removed from the oven prior to the radiosynthesis or alternatively stored in clean, dust-free sterile bags.
At various times during the digestion, vials are removed from the rotator and representative 100-μL samples containing both solids and liquid are removed from the well-stirred contents and diluted 18-fold into glass HPLC vials.
A 2 ml aliquot of the headspace gas from each vial was removed through the septum on the cap with a gas tight syringe and transferred to a 200 µl loop injection system on a Hewlett-Packard 5890 gas chromatograph (GC) equipped with a flame ionization detector.
Next, this vial was removed from the glovebox.
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