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The Onyx vials were placed on a shaker for at least 20 min to obtain a homogeneous solution before use.
The vials were placed on a temperature-controlled heating block.
Exetainer vials were placed on a 20-tube manifold and were evacuated for 1 min.
The sera vials were placed on a Symbiosis online SPE system (Spark Holland, Plainsboro, NJ) for the preconcentration of the analytes on a Polaris C18 cartridge (7 μm, 10 × 1 mm; Spark Holland).
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Approximately 1 mL of sample solution was transferred to 5-mL flat bottom borosilicate lyophilization vials (West Pharmaceutical, Lititz, PA), and the vials were placed on the shelf of an FTS DuraStop MP/DuraDry MP freeze-dryer (Stone Ridge, NY).
After incubation using an orbital shaker at 150 rpm, the GC vials were placed on the RSH Plus auto-sampler (Thermo Fisher) and injected onto a Trace 1310 gas chromatograph equipped with a flame ionization detector (FID).
Vials were placed on ice for 15 min and then centrifuged for 15 min at 4°C and 12,000 rpm.
On vaccination days, the vials were placed on wet ice no longer than 4 hours, and the vaccine formulated immediately prior to injection.
Vials containing 30-50 late third-instar larvae were placed on a rotating plate and exposed to a single dose of γ-radiation (10, 20, 30, 40 or 50 Gy) using a Cs irradiator (J. L. Sheppard and Associates, San Francisco, CA; Mark I unit, Model 30, Serial Number 668) with an average dose rate of 6.5 Gy/min.
Then, the vials were placed in a thermostatic bath (25°C) until a homogeneous media is reached.
The vials were placed, open, in a sealed plastic container containing silica-gel dessicant.
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