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All vials were analyzed after the experiment using an MD automatic analyzer (CMA 600, Kista, Sweden).
Acetaldehyde and ethanol in the sealed vials were analyzed by head-space GC using a Perkin Elmer Clarus 500 gas chromatograph with a Turbo-Mix HS 40 autosampler.
Vials were analyzed to confirm drug product identity and concentration as well as other product characteristics such as pH, crystal morphology, and particle size.
As only three vials were analyzed by each participant, the influence of the tube to tube variation may accidentally be smaller or larger resulting in corresponding smaller or larger uncertainties.
Analysis of variance of the CD4+ concentrations based on the order in which the vials were analyzed and the time elapsed between readings found no significant differences for either dilution 1 or 2 (Supporting Information Fig. S4).
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The amount of bortezomib present (assay) in the Bortenat 2- and 3.5-mg vials and VELCADE 3.5-mg vials was analyzed using a validated HPLC method.
For molecular analyses, 1 2 isolates from each vial were analyzed to avoid frequency biases attributable to potential germline jackpot events (Luria and Delbruck. 1943).
Each vial was analyzed in triplicate.
Only a single transposition male from each vial was analyzed.
Ten vials of each product were analyzed individually.
Paperpoints were collected in a vial containing RTF and were analyzed in the same manner as the intra-operative samples.
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