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After incubation, 75 µL aliquots of the reaction mixture solution in separate vials were added with 75 µL of Griess reagent (1.0% sulfanilamide and 0.1% naphthyl ethylene diamine dihydrochloride), mixed vigorously and incubated for 30 min in the dark at 25 °C.
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To the autosampler vials was added 25 uL of this solution.
Next, the dissolved content of these vials was added to the original solution A. The vials were then rinsed with the rest of solution B (10 mL for each vial) and solutions (A and B) were mixed together.
These products include: Chemical spikes, which are sold legally in small vials, are added to the urine sample at the time of testing.[15] Owning or purchasing the spike is not illegal, but adding it to your urine during your examination may violate local or state laws.
A competitive receptor-binding assay with [125I-Tyr4]-bombesin [125I-Tyr4]-bombesin [125I-Tyr4]-bombesininding affinity of BODIPY-BBN 5. PC-3 cells (5.0 × 105 cells per vial) wase added to vials containing medium (900 μL).
Typically, in preparing Au-Ag alloy, 30 mL vial was added to 2 mL of 10-mM HAuCl4 solution and 5-mL chloroplast solution, followed by the addition of 2 mL of 10-mM AgNO3 solution to the reaction mixture.
The content of one iTRAQ reagent vial was added to each sample, allowed to react and all samples were combined after labelling.
Then 0.5 ml from the first vial was added to the second vial containing HYNIC-TOC.
To a 20-ml microwave vial was added methyl 10-bromodecanoate (1.0 equiv).
For testing, an experimentally naïve WT target individual from a differentially labelled food vial was added to the group.
To this vial was added a solution of 2.5 mg of alkaline phosphatase (ALP) (or fluorescein-BSA) in 100 μL of water.
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