A series of vials were prepared with different concentrations of glycerol ranging from 10 to 200 g/l.
Then, 10 ml vials were prepared, stored at 4 °C and utilized during the following week.
Screw capped vials were prepared containing 1.0 mL solvent and 50 μg of quercetin (internal standard).
DNA was extracted, reaction vials were prepared for amplification, templates were added, and products underwent electrophoresis in separate rooms.
The randomization list was generated by computer, and NAC or placebo vials were prepared and numbered accordingly by the pharmacy.
The reaction vials were prepared in an atmosphere of N2(g) by using a N2(g -flushed AtmosBag -flushedldrich).
The glass vials were prepared by baking at 450°C to burn off all organic carbon, and the Teflon-coated caps were sonicated in methanol to remove any contaminants.
Water and sucrose, glucose, and fructose (all from Sigma) vials were prepared immediately before use by placing a circular piece of Whatman filter paper (GE Healthcare Bio-Sciences, Pittsburgh, PA) at the bottom of the vial to absorb 200 μl of liquid.
Transformation vials were prepared by mixing, (1) 12-μL cell suspension, (2) 5-μL boiled salmon sperm DNA, (3) ∼200- to 500-ng plasmid DNA, and (4) 45 -μL of 50% polyethylene glycol.
HCE (fig. 1) (Kind gift from Dr. Araki-Sasaki, K., Osaka University Medical School, Osaka, Japan) and Vero (National facility for animal tissue and cell cultures, Pune, Maharashtra, India) shell vials were prepared as per standard protocols.
