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For each phase diagram, cinnamon oil and specific Smix ratio were mixed thoroughly in different weight ratios (1 9, 2 8, 3 7, 4 6, 5 5, 6 4, 7 3, 8 2 and 9 1) in glass vials in order to delineate the boundaries of phases precisely.
The number of egressed cocoon clusters was noted and single cocoons were isolated into 1.5 ml vials in order to determine hatching success (i.e. the proportion of hatched eggs), brood size (i.e. the number of emerged adult offspring) and sex-ratio (i.e. the proportion of male offspring).
At the completion of the experiment, muscle tissue was dissected from the right posterior appendage and the abdomen (similar to above samples) and frozen in separate scintillation vials in order to determine if SIR values vary for these different tissue types.
Blood samples were collected in lithium heparinate-containing vials in order to prepare serum samples.
According to the approved and licensed release method used for ErA DP, reconstituted samples were pooled (approximately 20 vials) in order to provide enough sample for LO analysis.
Thus, we added eggs (of the same age) from the IV- bw population to each of our experimental hemiclone-producing vials in order to ensure a desirable density of 100 viable larvae per vial.
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Given the shortened incubation times along with the small volume of reagents, slide forceps were used to provide continued vertical agitation of the slides in each vial in order to facilitate reagent transfer during the dehydration process.
This approach involves the complete dissolution of the Y-labeled resin microspheres within the source vial in order to obtain a more homogeneous Y activity distribution followed by primary measurement of the triple to double coincidence ratio (TDCR) of the sample using both Cerenkov and liquid scintillation detection techniques.
Participants reported a mean of 22 (SD, 21) FVIII concentrate vials in each order.
As precautions, amber vials were used in order to minimize oxidation during analysis.
After providing written informed consent to the procedure, which was approved by the "LyonSud-Est2" ethics committee, subjects were taken to the test room, where they sniffed each vial in random order and rated compound pleasantness on a 9-point scale (from 1: "not at all pleasant" to 9: "extremely pleasant").
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