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Participants reported a mean of 22 (SD, 21) FVIII concentrate vials in each order.
From each of the LHst populations, we derived three sex ratio regimes – Male biased (M1-3, 24 males and 8 females in each vial, 19 vials in each replicate population), Equal sex ratio (C1-3, 16 males and 16 females in each vial, 14 vials in each replicate population) and Female biased (F1-3, 8 males and 24 females in each vial, 19 vials in each replicate population).
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Practitioner and patient independently designated by number the vial in each trial producing the weak response.
For each population, we separated males and females (as above) and set up six vials (with yeasted media) of males and six vials of females (∼10 flies per vial) in each of two blocks.
Each trial used unique pairs of visually identical, randomly numbered, sealed vials; one vial in each pair contained saline solution; the other contained saline solution plus ionic hydroxylamine hydrochloride (NH3OH)+.
Because the average snakebite victim needs two doses, for 300,000 annual patients, "we probably need 600,000 vials in Africa each year," he said.
Half the males were placed in vials in groups of four, each group with 20 female flies that were ready to mate, allowing the males to mate with multiple females.
50 0-4-hours-old males and females of each genotype were placed into separate vials in groups of 25 for each gender.
Logarithmic dilutions of 500 μl of C. trachomatis cell culture controls from 10-3 to 10-7 were added to the shell vials in rows of 4 for each dilution.
Logarithmic dilutions of 500 μl of C. pneumoniae cell culture controls from 10-2 to 10-5 were added to the shell vials in rows of 4 for each dilution.
Subsequently, the diluted suspension was aliquotted into 4 vials, (81 μl in each vial).
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