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Purified samples were placed into a 96-well plate holder or single vial holder of an Agilent 1200 Series HiP-ALS (High Performance Automated Liquid Sampler, G1367B) and sequentially injected by 0.8 ml/min flow from an Agilent 1200 Series Isocratic pump (G1310A).
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Also, self-made vial holders (designed to hold vials in a 10 × 10 array to match the dispenser) were used to facilitate the work.
After drying, the virus-contaminated discs were transferred into 25 ml plastic vial holders (Sarstedt AG & Co. KG, Nümbrecht, Germany), which were previously filled with 0.5 g of glass beads (0.25 0.50 mm diameter, Carl Roth GmbH, Karlsruhe, Germany) to increase virus recovery by mechanical abrasion.
Therefore, as soon as a fly was inserted into the holder, the next vial was put on ice in order to avoid unnecessary delays.
Analyses involving aqueous suspensions of water-soluble lens proteins were conducted in cylindrical optical glass specimen vials positioned in a rigid holder mounted to the instrument.
Vials were held in a plastic holder tight to the bottom of an ultrasonic bath WUG-AO2H (Wise Clean Company, Korea) at 10°C and sonicated for 1-2 h at an ultrasound frequency of 28 kHz and an intensity of 0.84 W cm−2.
The vials were placed in the UPLC sample holder.
To the right are three microphones, an exercise ball in a holder, a side table housing various vials, and a few floor pillows.
177Lu vials were put at the bottom of the dedicated syringe holder raised at 3 cm relative to its normal position in order to place the vials closer to the area of highest sensitivity.
The solid-phase assembly holder, 75 μm carboxen/polydimethylsiloxane (CAR/PDMS) fiber, butyl rubber septa, 20-mL glass vials and aluminum vial crimp seals were supplied by Supelco Inc. (Bellefonte, PA, USA).
The vials are positioned in a plastic well-plate format sample holder.
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