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Afterwards based on the obtained relation, eight design parameters in milling, namely, number of balls, ball diameter, vial radius, vial height, ball diameter distribution coefficient, plate spinning rate, vial spinning rate and distance between the center of the plate and the center of the vial were optimized.
Based on Burgio׳s model eight design parameters in milling, namely, number of balls, ball diameter, vial radius, vial height, ball diameter distribution coefficient, plate spinning rate, vial spinning rate and distance between the center of the plate and the center of the vial, were optimized by the PSO algorithm.
Twenty individual flies were gently introduced into a glass vial height 240 mm tall and 25 mm in diameter.
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Experimental matings were staged in food vials (height: 8 cm, diameter 3 cm) stood upright and held at room temperature (18 ± 1°C) under continuous lighting provided by light bulbs (11W/827) suspended ca 40 cm above the vial rims.
In order to maintain a high moisture content in the food, we provided fresh grass in small plastic vials (height x diameter: 5.8 cm × 2.1 cm) filled with water, and replaced the grass every other day in order to avoid withered or yellowing grass.
PS solutions were added to 10 mL portions of diluted S. aureus or E. coli in flat-bottom 2.5 cm diameter vials (solution height in the vials was 2 cm) and incubated for 15 min in the dark.
From each standardised population, eggs laid during a 2 h window (ZT01 03) were collected and exactly 30 eggs were dispensed into each long vial (18 cm height × 2.4 cm diameter) containing ∼6 ml BJ medium.
Thereafter, they were allocated individually to plastic vials (7.5 cm height, 3.5 cm diameter) filled to a height of 6 cm with dechlorinated tap water.
From each replicate population, ∼300 eggs were transferred into glass vials (18 cm height × 2.4 cm diameter) containing 10 ml of BJ medium.
Three days later, eggs were collected over a 3 h window on BJ medium and approximately ∼300 eggs were transferred into glass vials (18 cm height × 2.4 cm diameter) containing ∼10 ml of BJ medium.
All experiments were conducted at 25° and in a humidified constant temperature room (∼50% relative humidity, RH), using glass vials (75-mm height × 25-mm diameter) containing 7 ml of SY food with ad libitum live yeast granules or live yeast paste added.
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