The two purified proteins were mixed in a weight ratio of 1∶1 in a vehicle of PBS with 0.01% Polysorbate 20® and aliquots dispensed into vials for storage at +2°C to +8°C.
After clotting, serum was separated (centrifuged at 2000 g) and aliquoted to polyethylene Eppendorf vials for storage at −80°C until analysis.
Stool samples collected during the visit or provided within 48 h and kept cool were homogenized and transferred into multiple vials for storage at –80°C.
Blood samples will be kept on ice, centrifuged at 3000 rpm for 10-min, within 6 h of collection and the plasma transferred to a labelled cryo-vial for frozen storage (at −20°C or lower for short term storage).
There, the medical technologist prepared aliquots of each subject's serum and transferred these aliquots to vials for frozen storage, following a standard operating procedure (SOP).
Peripheral blood plasma was separated by centrifugation (15 min at 2,000× g) and aliquoted into individual vials (100 200 µl) for storage at −80 °C prior to the study.
Vials are transported on ice for storage in -70°C freezers.
Vials were transferred to -80°C for storage pending analysis.
Swarf was collected into paper bags and allowed to dry to ambient moisture levels in an air conditioned room for at least 48 hours prior to transfer into plastic vials (10 ml Falcon tubes) for storage and shipment.
All tissues were separately washed quickly with 50 mL of ice-cold saline (0.9% NaCl) and placed into cryogenic vials that were dropped into liquid nitrogen for storage.
