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Consistently, as shown by the viable stain propidium iodide, the viability of the MOIL that we isolated was > 94% (propidium iodide negative).
When interpreting genetic inactivation data it should, however, be recognized that the inability to isolate a viable stain under standard laboratory conditions is generally judged as evidence of essentiality, albeit these conditions might not reflect the growth conditions in, e.g., the host environment.
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Viable staining of the mitochondrial marker MitoTracker red (red fluorescence) in A549 cells excluded the possibility of C-1330 localination in mitochondria (Supplementary Figure 2).
GFP-tagging of proteins provides viable staining of different cell structures and organelles and analyses of subcellular protein localization [ 6, 7].
Viable cells stain green, while apoptotic cells stain green and shrunken with condensation of the nucleus, the necrotic cells stain red [18].
Dead cells stained red and viable cells stained green.
The use of a combination of Cyto 82 as viable cell stain (green) and PI for identification of damaged or dying cells (red), again demonstrated a very similar pattern of lysing cells and viable cells in vegetative hyphae of M145 and FM145, again illustrating the wild-type growth behaviour of FM145 (Figure 2i j).
Since lethally damaged cells do not retain these reactants, nonviable areas remain unstained and appear pale, whereas viable cells stain red.
The cell and scaffold construct was evaluated using Hematoxylin and Eosin (H&E) staining as well as fluorescein diacetate (FDA) viable cell staining.
We assessed whether intracellular bacteria at day 5 were viable by staining cells with the LIVE/DEAD BacLight Kit which differentiates live and dead S. aureus by staining the latter with a red fluorophore and viable bacteria with a green fluorophore (Fig. 3D).
Unstained (viable) and stained (non-viable) cells were counted separately in a hemacytometer and total number of viable cells in the population was calculated.
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