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In general, 50%% or more viable spores were recovered, with ascospores of X. elegans showing the highest survival.
Several hybrids are known, but all are sterile as there is no doubling of the chromosome number to allow chromosome pairing and consequent production of viable spores.
The experimental response was log (N0/Nt), where N0 and Nt (spore ml−1) the concentrations of viable spores at t = 0 and t respectively.
Only the live viable spores, separated from dead spores under a stereo microscope, were used for DNA extraction.
These strains were capable of mating with wild-type haploids to produce diploid progeny capable of undergoing meiosis to produce four viable spores.
In one instance, a complete secondary proliferation was noted (i.e., growing from a primary proliferation) that developed completely so as to produce viable spores.
Only colonies with viable spores from both asci were included for analysis.
Each of the normal tetrads had two viable spores while no spores from abnormal asci were viable (data not shown).
The mean number of viable spores in toxic leaf litter was about 400 times that in non-toxic samples.
As a result, the reduced pathogenicity of photocatalyzed spores could not be simply attributed to the reduction of viable spores.
Tetrad analysis revealed two viable and two unviable spores, all the viable spores being kans (data not shown).
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