Concomitantly, the oxidative stress-mediated cell-death was ameliorated and the viable-cells' percentage was enhanced following DOR activation.
Viable cell percentages were determined by the FACS Calibur apparatus and the Cell Quest Pro software from Becton Dickinson (BD Biosciences).
The MTT-assay was used to evaluate the CE effect on HepG2 cell viability, and the results were expressed as viable cell percentages with respect to the control.
Therefore the percentage viable, apoptotic and necrotic IPEC-J2 cells was determined using flow cytometry.
Results are expressed as percentage of viable and non-viable cells.
Cell survival was presented as percentage of viable cells out of the corresponding viable cell number in untreated controls.
Cytotoxicity was quantified as the percentage of viable treated cells relative to the viable cells in an untreated control.
Results were expressed as percentage of viable cells, considering control cells incubated with medium alone as 100% viable.
h Percentage of viable cells after overnight incubation.
Variables evaluated in each pollination assay were the percentage of viable seeds produced and percentage of empty seeds.
