A new protocol specifically designed to enumerate total viable bacteria in milk powder using flow cytometry was validated against aerobic plate counts using 178 samples of whole milk powder.
However, when identifying viable bacteria in pasteurized milk, commercial PCR primers designed for detecting long stretches of DNA are generally not available.
Total viable counts (TVC) of milk from individual camels increased significantly from 4.91 ± 1.04 at production to 6.49 ± 0.77 at secondary collection centres and 7.52 ± 1.32 at the Nairobi market.
It is applied to vulnerability to failure of a batch-continuous pasteurizer for raw milk containing viable Mycobacterium avium subsp. paratuberculosis (MAP) - a chronic enteric pathogen linked to Crohn's disease in humans.
The reported high excretion of viable T. gondii in milk of naturally infected buffaloes from Iran by Dehkordi et al. [ 4] needs confirmation.
Recently, Dehkordi et al. [ 4] reported isolation of viable T. gondii from milk of 7 of 164 buffaloes in Iran, but the validity of these results has been questioned [ 5].
For these and other reasons, there is a steady increase in the consumption of fermented dairy products (i.e. yoghurt and other fermented milks) containing viable LAB.
The protocol was then used to enumerate total viable bacteria in samples of milk and whey collected at different stages during the manufacture of milk powder and whey protein concentrate.
Additionally, an increase in viable MAP isolated from retail milk in the summer has been shown [ 21].
Ideally, it is best to get your protein from natural sources, but if that is not possible, whey, soy, and powdered milk are all viable options.
