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The percentage of viable isolated cells determined by trypan blue exclusion was greater than 90%.
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Obtaining viable isolates appeared to be limited by the efficiency of current immunomagnetic separation (IMS) methods, which ranged from 20 to 100% effectiveness at retrieving colonies depending on serogroup.
Unfortunately, there is no mechanism to recover viable isolates from these fixed organ culture samples but we have been able to establish the presence of Candida by specific staining with a rabbit polyclonal antibody (Fig 5 B, D).
Attempts of deleting the chromosomal rpoN gene yielded viable isolates.
These PI-2 containing PI-2 containingted for ≈3.6% of all visolatesolaccountedected in 1999.
Overall, 21% of all viable isolates collected in 2006 contained PI-2.
The estimation of total viable isolates in 1999 was based on the known proportion of viable isolates to total collected isolates of the PI-2 containing PI-2 containing99 (93%).
PFGE profiles of the 11 viable isolates from the biobank and a control strain ATCC 19615 were performed.
Colonies of viable isolates were extracted, amplified and detected via electrophoresis to determine the specific genetic patterns.
Of the 125 patients with viable isolates, majority 116 (92.8%) were newly diagnosed and 9 (7.2%) had previous history of TB treatment.
Persons excluded from analysis because there was no viable clinical isolate of M. tuberculosis did not differ statistically from those with viable isolates by age (P = 0.44), gender (P = 0.56), or race (P = 0.70).
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