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Some of these markers could only have been identified thanks to the extent of the genetic variability which the GWA approach makes accessible; following a validation exercise, they may well prove to provide viable indirect selection tools in a practical breeding programme.
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Indirect counting of viable cells was carried out by CCK-8 assay using a Cell Counting Kit-8 (Dojindo, Rockville, MD, USA).
After treating melatonin and/or LPS, the fixed area (10 mm) at the center of each well was converted into a digital image using a digital still camera (Olympus, Tokyo, Japan) and the number of neurospheres whose diameter was over 60 μM was counted by Image J. The indirect counting of viable cells was carried out by WST-8 assay using a Cell Counting −8 kit (Sigma, MO, USA).
Furthermore, ADCdiff conveys indirect information concerning the viable part of the tumours and the condition of the tumour microenvironment [26].
Culture supernatants from wells with viable clones were screened by an indirect ELISA using recombinant CA XII protein.
Viable clones were screened by an indirect ELISA using 96-well microtiter plates coated with PCV2-Cap VLPs.
Although more laborious, this cell counting/cell viability assay was favored over the Cr release assay (as well as MTT assay) since this method allowed for direct assessment of viable cell numbers, unlike methods using indirect measures of radioactivity release.
The results of this research suggested that microfluidic ITP with indirect fluorescence detection is a viable technique for separation of organic acids in human serum samples, especially when a chip with suitable design is used.
Processed frozen bone allografts lack viable donor cells and may trigger an indirect pathway of alloantigen recognition in the recipient.
Sera from mice immunized against each of the tumours were tested against viable cells of the immunizing tumour by indirect immunofluorescence.
To determine the level of anti-vaccine antibodies, sera were used in an indirect immunofluorescence assay to stain viable vaccine cells followed by cytofluorometric analysis as previously described [ 3].
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