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Determination of the O H serotype and intimin subtype of 29 of the 30 EPEC strains (1 was not viable) indicated that they were highly heterogeneous (Table 3).
Furthermore, the HSC derived from sorting were highly viable indicated by 89.3% living cells, similar to that after the iohexol gradient as retrieved from trypan blue staining (90%) (detailed data not shown).
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The four Sans alleles described here are homozygous viable, indicating that CG13320 (Sans) is not essential for viability.
Additionally, H-ras knockout (KO) mice were found to be viable, indicating that H-ras is dispensable for embryogenesis [6].
Mice deficient in both SphK1 and SphK2 are not viable, indicating an essential cellular requirement for S1P in addition to its role in inflammation [16].
So far, H-ras knockout (KO) mice were found to be viable, indicating that H-ras is not required for embryogenesis [3].
Indeed, elav-Gal4>UAS-Rheb animals are viable, indicating this degree of pathway activation is considerably more mild than loss of Tsc1 (see below).
The triple mutant, ΔrnhA ΔrnhB Δfen1, was viable, indicating that their function in DNA replication is not essential for cell growth [121].
Mice deficient in either AMPKα1 [36] or AMPKα2 [37] are viable, but mice deficient in both α1 and α2 are not viable, indicating that the two isoforms have partially redundant functions.
In experiments where FK866 treatment (33 nM) was extended for up to 12 days, unstimulated PBLs were still >80% viable indicating that resting T cells are mostly resistant to FK866-induced cell death (data not shown).
All genetic combinations were viable, indicating that the synthetic lethal relationship between rps15-1 anhp2ins9ns9 cannot be explained by the absence of the snR36 and snR85 guided modifications in the vicinity of Rps15p's binding site.
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