The 16-mg/kg dose was considered to be the maximum viable dose.
The dose of AS1402 would not be increased beyond 16 mg/kg, as this was considered to be the maximum viable dose.
The innovative clinical trial designs such as adaptive or seamless studies compress timelines, improve dose and regimen selection, and reduce the number of patients assigned to non-viable dosing regimens.
At 24 h.p.i., significantly higher numbers of neutrophils were observed in the hindbrain in larvae injected with low-dose viable, high-dose viable and high-dose non-viable spores compared with PVP control injections (P<0.001).
At 24 h.p.i., significantly higher numbers of macrophages were observed in the hindbrain in larvae injected with low-dose viable, low-dose non-viable and high-dose viable spores compared with PVP control injections (P<0.001).
This is achieved at therapeutically viable siRNA doses without cytokine induction, toxicity, or off-target effects associated with the use of unmodified siRNA.
Significantly higher numbers of neutrophils were found at the site of infection 4 h after injection of a low or high dose of viable or a high dose of non-viable spores compared with the PVP control injections (P<0.001) into the hindbrain ventricle.
No significant macrophage recruitment was seen at this time after injection of low-dose viable or non-viable spores and high-dose non-viable spores.
At 24 h.p.i., significant levels of il1b were transcribed after a high-dose viable spore challenge (P<0.001) and of tnfa after low- and high-dose injection of viable spores (P<0.05 and P<0.001, respectively; Fig. 4A,B).
To therefore ensure a constant number of viable cells, repeat doses with smaller cell numbers or scatter injection points may benefit local MSC administration.
Adult H. ligniperda were more tolerant of radiation with 1.6% of adults producing viable eggs at doses of between 100 and 150 Gy despite 100% sterility being recorded at 75 and 175 Gy.
