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In marine culture, Ganesan et al. [ 3] studied the viable cultivation of H. musciformis by coir rope on the southeast coast of India.
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The methodology of analysis for the viable, cultivable, airborne microorganisms is based on the cultivation of the air sampled microorganisms on specific microbiological growth media.
It must be mentioned, that the choice of specific selective or non-selective microbiological growth medium, the incubation temperature and the cultivation time result to differences on the selection of the viable, cultivable, airborne microbial community.
In addition, cells in a packed-bed remain much longer viable during cultivation in an easy to operate system.
Although these plants exhibit slow development and reduced vegetative and floral organs they are apparently viable in cultivation.
By contrast, significantly more islets were seen to be viable following cultivation under rotary conditions or when assessed immediately after isolation.
Regardless of the culture media, most of the viable cells, after cultivation with IL-2 supplementation, were T lymphocytes, while monocytes and B lymphocytes were not detected.
The prevalence was measured as viable counts in cultivation on supplemented MRS (2% fructose and 0.1% l-Cysteine).
Co-cultivation, viable counts, gentamicin assay, electron microscopy and statistical analysis showed that co-cultivation of wild type and luxO mutant of V. mimicus strains with A. castellanii did not inhibit growth of the amoeba.
More than 97% of the cells in chemostat culture represented viable cells throughout all cultivations (Table 1).
Thus, it indicated that the post EGAS tissue remains viable throughout the EGAS cultivation.
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