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Regarding, the overall growth and toxin production in the tested laboratory media, it is palpable that the viable counts of B. sphaericus strains varied according to growing medium, P medium had the highest viable counts for all strains throughout the cultivation time (Figure 9).
Differences in the total viable counts for each isolate were tested for by one-way analysis of covariance (ANCOVA), with Tukey, Bonferroni and Duncan post-tests being used for multiple comparisons.
Viable counts for each isolate were calculated every second day.
Viable counts for pure S. cerevisiae cultures were generally higher than in mixed cultures (Fig. 2).
In the analyses here presented for the studied strain 235/02, no lysis took place, that is: neither cytoplasmic proteins were identified, nor lower viable counts for protease-treated cells were found.
Viable counts for the unadapted cells dropped below the detection limit (<50 CFU/ml) after the challenge, indicating that these cells were sensitive to this high concentration of bile salts.
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FTIR spectra were obtained from the surface of beef samples, while microbiological analysis determined the total viable count for each sample.
At 37°C, the level of Salmonella in egg white remained relatively unchanged for 16 h, but after six days the counts of STM had dropped by one log10 and the viable count for RS had dropped below the limit of detection (5 CFU/mL), while SS counts remained unchanged.
Data for viable counts of the microorganisms were normalized by logarithmic transformation, and nontransformed values were presented in the Results.
Furthermore based on the guideline for hand hygiene in health-care settings a minimum of 5 log10 reduction of viable counts of microorganisms must be achieved for a successful disinfection [ 100].
Lavage fluid was collected from the nares for determination of viable counts of bacteria in serial dilutions plated on selective medium, containing antibiotics to inhibit the growth of contaminants.
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