Sentence examples for viable conditional from inspiring English sources
A global assessment of transcriptome changes in adult brain lacking multiple APP family members (a tissue more relevant for AD) will await the generation of viable conditional mutants.
We recently generated a viable conditional knockout mouse model to delete xbp1 in the nervous system using the Nestin promoter to express Cre (XBP1Nes−/−), which do not present basal motor phenotypes.
It will therefore be of high interest to analyze phenotypic and gene expression changes in adult APP/APLP2 double or APP/APLP1/APLP2 triple deficient brain, once viable conditional combined mutants become available that are currently generated by crossing mice with floxed APP and APLP2 alleles with transgenic tissue-specific Cre mice [ 56].
As previously described, we generated viable IRE1α conditional KO mice (Mox2+/Cre; IRE1αΔNeo/ΔR) and control mice (Mox2+/+; IRE1αΔNeo/ΔR) by breeding Mox2+/Cre; IRE1α+/ΔR mice with Mox2+/+; IRE1αΔNeo/ΔNeo mice [18].
In this study we used novel viable ADAM10 conditional knockout (ADAM10 cKO or A10 cKO) mice with specific postnatal deletion of the protease in forebrain neurons (Prox et al., 2013).
Therefore, we used somatic clustered regularly interspaced short palindromic repeat (CRISPR) to create mosaic viable and conditional knock out of various genes (Jinek et al., 2012; Cong et al., 2013; Shen et al., 2014).
In the construction of the BFAN collection these knock-out strains were not viable and therefore conditional mutants have been made using a pMUTIN3-vector.
In a typical SGA experiment, a query strain, carrying a mutation in a gene of interest, is crossed to an input array of viable deletion mutants or conditional alleles of essential genes.
Wls conditional homozygous mice are viable and fertile and do not show any abnormalities.
Cre-positive (all three drivers)/ Wt1 conditional heterozygous mice were viable and healthy (data not shown).
ErbB2-deficient conditional mutant mice were viable; however, physiological analysis revealed the onset of multiple independent parameters of dilated cardiomyopathy.
