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The number of viable cells was determined by plating out serial dilutions on TSBA.
A greater percentage of viable cells was also achieved through dounce homogenization.
The plate containing viable cells was washed twice with PBS.
Twenty-four hours after incubation with complexes, the percentage of viable cells was calculated.
The OD value of viable cells was measured by cck-8 assay.
After 63 days, the fraction of viable cells was 53.7%, while 46.1% stain red and thus are dead (SEM = 2.3).
The number of viable cells was determined by CFU counts as described in the Materials and Methods section.
The confluent layer of viable cells was formed on both the reference surfaces, Si and Si + Au (Fig. 6a, b).
The MTT-formazan formed by metabolically viable cells was dissolved in 100 μL of SDS for 12 h.
The percentage of viable cells was calculated as a ratio of absorbance at 490 nm of treated to control cells.
After the end of the incubation period, the number of viable cells was determined by the MTT test.
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