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Maximum viable cell concentrations, viable cell yield and productivity obtained were significantly different (P < 0.05) at all of the three agitation speeds studied.
Maximum viable cell concentration, viable cell yield and viable cell productivity obtained were significantly different (P < 0.05) at both with and without pH control conditions.
Maximum viable cell concentration, viable cell yield and productivity obtained were significantly different (P < 0.05) at both facultative and anaerobic conditions.
Viable cell yield and viable cell productivity obtained were significantly different (P < 0.05) at all of the three feeding rate studied.
The granular cells cryopreserved under these conditions exhibited a cell viability of 82.7 ± 2.7% and a viable cell yield of 28.6 ± 2.2%.
Our results indicate that the initial addition of 8% dimethyl sulfoxide (DMSO) and seeding of extracellular ice do not significantly decrease viable cell yield.
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Cell proliferation and death were not explicitly measured, thus reductions in viable cell yields cannot be specifically attributed to cytotoxic or cytostatic mechanisms.
Cell viability at harvest was >85% for all cultures, but viable cell yields were lower for treated vs. control (data not shown).
After incubation of the cells at 37 °C, various concentrations of the sample were added, and the incubation was continued for 24 h and viable cells yield was determined by a colorimetric method.
Similar to the results of maximum viable cell concentration, yield and productivity, the same trend was obtained for lactic acid production, yield and productivity, where the results were slightly higher in facultative condition (12.98 g/L, 0.91 g/gGlucose and 0.53 g/L h respectively) compared to anaerobic condition (11.32 g/L, 0.86 g/gGlucose and 0.46 g/L h respectively).
There were significant differences (P < 0.05) in maximum viable cell concentration, yield and productivity for both fed-batch fermentations with and without resin addition.
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