Your English writing platform
Discover LudwigSimilar(60)
For clonogenic assays using OCM1A and 92.1 cells, flow cytometry (MoFlo; Cytomation) was used to seed one viable cell per well in ultra-low attachment 96-well plates containing MDMF medium as previously described [ 23].
ALDH+ and ALDH− cells were isolated from Melmet 1 and Melmet 5 xenografts by FACS, distributing one viable cell per well in hESCM into 96-well plate.
Cells were washed in DMEM/F12 medium and seeded at 5,000 viable cells per well in a 96 well plate with triplicate wells per condition.
3000 viable cells per well into 96-well tissue culture plates in a final volume of 100 μl.
PHCs were plated at 200,000 viable cells per well in 6-well culture plates 24 hours prior to siRNA transfection procedure.
Melanoma cells were plated at a density of 2 4 × 10 viable cells per well in 96-well plates and cultured for 2 d in 100 μl SCM with vehicle (0.02% DMSO) or with PN at indicated concentrations.
The lymphocytes were then counted and seeded at 1 × 10 viable cells per well in round-bottomed 96-well micro-well plates with RPMI 1640 medium supplemented with reduced PHA-M (1 μg/mL), 10% human T-stim (Collaborative Biomedical Products, Bedford, MA, USA), 20% HL-1 medium, and 15% BCS.
C. albicans were added to each well at 200 viable cells per well in a 37 °C/5% CO2 incubator.
Cells were seeded into 96-well plates at 104 viable cells per well and left to attach to the plate for 24 hours.
Cell lines were seeded into 96-well plates at 10 viable cells per well and left to attach to the plate for 24 h.
Roughly, 3 × 10 to 4 × 10 viable cells per well were placed into six-well plates in a volume of 3 ml and incubated for 3 hr.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com