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The initial viable cell number was 2.107-1.108 cfu/ml.
The results found are precise and can be correlated to the number of viable cell number.
They are rapid and convenient in determining viable cell number in proliferation or cytotoxicity studies.
Simultaneous decline in viable cell number and larval weight validates the result of MTT assay.
The viable cell number of MSH1 beads was 3 6 × 109 CFU g−1 with an average of 4.4 × 109 CFU g−1.
As seen in Figure 1a, the initial viable cell number was similar for pure, heated and waste oil (about 4.107 cfu/ml).
The rate of viable cell number decrease in the presence of the Natto peptide was lower in P103 than in the parent strain R6 (Fig. 3b, c).
When the cells were immobilized using nata and freeze dried, it was observed that viable cell number decreased from 109 – 1010 CFU/g to 107 CFU/g.
Subsequent metabolic engineering to overexpress MDH II in CHO resulted in increases in intracellular ATP and NADH, and up to 1.9-fold improvement in integral viable cell number.
Results showed that spatial distributions regularities of volatile suspended solid (VSS), respiration intensity, protein, polysaccharide, viable cell number and dehydrogenase activity were consistent with the aqueous rate.
Absorbance levels were then translated to the viable cell number using a previously prepared calibration curve.
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