CellTiter-Glo kit determines the number of viable cells based on the quantitation of adenosine triphosphate, which signals the presence of metabolically active cells.
At various time intervals after the treatment, the numbers of trypsinized cells were determined using a Coulter Counter simultaneously with the determination of metabolically active viable cells based on determination of intracellular ATP in cell lysate.
Cell viability was determined by cell counting kit-8 (CCK-8) assay (Dojindo, Japan), which measures the number of viable cells based on bioreduction of a water soluble formazan.
Following gel polymerization, the hMSC laden hydrogels were cultured under standard conditions, and cell viability was assessed at 24 and 72 h via Live/Dead staining, which discriminates dead cells from viable cells based on membrane integrity.
Cell survival was assessed via the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, Southampton, UK), a colorimetric method for determining the number of viable cells based on a novel tetrazolium compound, inner salt (MTS).
Cytotoxicity was measured using the Cell Titer 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA), which is a colorimetric method for determining the number of viable cells based on bioreduction of the tetrazolium compound MTS (3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2 H-tetrazolium, inner salt) by metabolically active cells.
The mean number of viable cells based on trypan blue exclusion (for all 5 donors) immediately after thawing was 10.6 ± 3.3 × 10 per vial, with a minimum of 4.3 and a maximum of 18.1 × 10 cells being reported by the participants.
The Guava ViaCount assay distinguishes between viable and non-viable cell based on the differential permeability of DNA-binding dyes in the ViaCount reagent, and thus fluorescence of the dyes allows quantitative assessment of both viable and non-viable cells in suspension.
The CellTiter 96® AQueous one Solution Cell Proliferation Assay (Promega) is a colorimetric method for determining viable cell number (based on the MTT assay).
Viable cell counts, based on trypan blue exclusion, were conducted using a hemocytometer.
The reagent ALDEFLUOR™ that is frequently used for viable cell sorting based on ALDH activity has only been validated for ALDH1 and ALDH3 and not for the remaining 17 ALDH enzymes [ 17].
