Sentence examples for viable by using from inspiring English sources
We further show evidence that, after the loss of culturability, the cells remained viable by using a variety of criteria, including cellular membrane integrity, uptake and incorporation of radiolabeled amino acids, and protection of genomic DNA from DNase I digestion.
Mobile telemedicine services with video capability have become viable by using smartphones [ 30, 33].
Such conditions can be expressed in terms of linear matrix inequalities, allowing for a viable design by using convex optimization.
Ten microlitres from this solution were used to count the viable cells by using a Countess automated cell counter (Invitrogen, UK).
In order to examine the role of ceramide synthase activity in this process, we adapted a previously described protocol to analyze ceramide synthesis in viable cells by using d17 : 0 dihydrosphingosine and mass spectrometry.
The survival of cells was quantified as the number of viable cells by using a colorimetric method (CellTiter 96 AQueous One Solution Cell Proliferation Assay; Promega) that quantifies the formation of a formazan product from 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfophenyl -2H-tetrazolium (MTS) in live cells.
Briefly, the optical absorbance of each well at a wavelength of 450 nm was measured to estimate the production of formazan, which reflects the number of viable tumor cells, by using a SpectraMax Plus 384 Microplate Spectrophotometer (Molecular Devices Japan, Tokyo, Japan).
The viable methods are therefore by using time-of-arrival measurements which require strict synchronization amongst all the nodes or by two-way ranging.
Distribution of apoptotic, death and viable cells were determined by using Annexin-V-PE Annexin-V-PE Annexin-V-PE Apoptosis to the manufacturers instructions (Bdetectionnce, San Jose, CA).
Number of viable cells was determined by using trypan blue dye.
The cell pellet was resuspended, and viable cells were counted by using trypan blue dye exclusion.
