The condition of the quality of the brood pattern was also noted with areas of capped brood containing less than 80% viable brood (as indicated by cells empty of brood) were considered "spotty" while those brood patterns that had less than 20% brood mortality were considered "solid".
Introduction of any Rfa2 N-terminal mutation by plasmid shuffle resulted in the formation of viable cells, as indicated by growth on 5-FOA.
After five days in culture without serum supplementation, explants retained fully viable chondrocytes as indicated by the abundant green staining and lack of red staining in the controls.
For each CD31-labeled section of control and treated tumors, five microscopic fields (200× magnification) containing exclusively viable tumor cells as indicated by hematoxylin staining were selected randomly for analysis.
For each section, 10 fields containing exclusively viable tumour cells, as indicated by the haematoxylin staining, were selected randomly for analysis.
For each GSL-1 or MIB-1-labelled section of control or CMDB7-treated tumour, five fields containing exclusively viable tumour cells, as indicated by the haematoxylin staining, were selected randomly for analysis.
Fig. 4 Demonstration of intact pine cone (week 104) microCT scan in slice (a) and 3D virtual cut-open (b) images, with non-viable seeds upper left (1) and viable seeds top right (2) as indicated by their voids.
Cells were plated (5 × 10 cells per 60-mm dish) and viable cell numbers were counted as indicated time using BrdU cell proliferation kit according to the manufacturer's manual (Cell Signaling Technology, Inc .. Invasion assays were performed as previously described using Matrigel invasion assay Kit (BD Bioscience, Inc., Franklin Lakes, NJ, USA).
Moreover, employed TMPyP-mediated treatment resulted in 4 log reduction in viable counts and not >6 logs, as indicated for fulleropyrrolidine.
Pollen was viable in all individuals that produced it as indicated with the in vitro test.
