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Viability (verified before every experiment) was >99% and PMNs constituted 90 99% of leucocytes.
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Conversely, for CdS samples, as previously reported in the literature [62, 63], an adverse reduction of approximately 30% of cell viability response (~70% viability) was verified, which was attributed predominately to the potential cytotoxicity caused by release of Cd2+.
In control experiments, cell viability is verified by the addition of a two-photon fluorescence channel to the OCM.
In all cases the absence of viability was verified using plate culture, followed by incubation at optimal conditions.
Cell viability was verified by 7-amino-actinomycin D (7-AAD) or Propidium Iodide (PI) and Annexin-V staining (BD Biosciences).
None of the diluted supernatants affected cell viability, as verified by the trypan blue exclusion test.
Viability was verified by the presence of a fEPSP, and facilitation of fEPSP.
Cells were counted with a hemocytometer and viability was verified by trypan-blue (Sigma-Aldrich, Taufkirchen, Germany) exclusion.
Similar transfection efficiency (50%) was assessed by FACS analysis and cell viability was verified using trypan blue.
To ensure that gene suppression was not due to CO cytotoxicity, cell viability was verified by quantifying live cells using a nucleocounter (New Brunswick Scientific, Edison, NJ).
This reduced miR-21 expression did not result from a decrease in cell viability as verified using calcein staining (Figure S1A).
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