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Figure 2 illustrates a time course cell viability pattern, showing acute versus chronic exposure to arsenic trioxide.
Arrivals varied significantly among subpopulations (Table 1b), but the pattern (Fig. 2B) did not match the pollen viability pattern.
This loss of viability pattern is obscured in the transcriptomic response to slower acting anti-malarial drugs such as pyronaridine and chloroquine [ 15] and antifolates [ 3], perhaps because of confounding transcriptomic changes occurring through normal cell cycle progression during the longer drug treatment period needed to observe a significant transcriptional response.
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Despite the large number of meioses with nonexchange chromosomes in the msh4 –R676W hypomorph, analysis of spore viability patterns suggests efficient segregation of natural nonexchange chromosomes in S. cerevisiae.
The effects of transfection were subsequently assessed on cell viability patterns, cell migration and alterations in gene and protein expression by real-time quantitative PCR and immunocytochemistry respectively.
In higher plants, they play a structural role in cell viability, embryogenesis, pattern formation, cell division, chloroplast biogenesis, and modulation of activity and distribution of membrane-bound proteins such as enzymes and receptors [ 2, 3].
Though the enhancement ratio achieved with this particular gold enhancement reagent is modest, this is a clear demonstration of the viability of patterning and drying reagents for dry reagent storage in paper-based assays.
Though this is just an initial proof-of-concept demonstration of the viability of patterning reagents for dry storage and subsequent rehydration, it is useful to compare these values with published limits of detection for a variety of malaria diagnostics (Table 1) to benchmark whether this sensitivity is even within a relevant clinical range.
To show the viability of patterning and drying reagents directly onto a paper device for dry reagent storage and subsequent controlled release, we tested this device with the malaria antigen Plasmodium falciparum histidine-rich protein 2 (PfHRP2) as an example of target analyte.
In addition, ppa009789m was very similar to RUPTURED POLLEN GRAIN1 (RPG1), a component of the MtN3/saliva gene family coding for a plasma membrane protein essential for microspore viability and exine pattern formation in Arabidopsis[ 30], even though they could not be considered as putative orthologs by RBA (Table 1).
The relatively higher patterning viability is due to reduced droplet packing density, which minimizes possible exposure to mechanical shear forces at the valve during cell encapsulation.
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