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The procedure for assessing the viability of the brain slices after the 5 h incubation period is also described.
For evaluation of the effects of changes in viability of the brain slices during the incubation, aECF samples can be taken at different time points (after 1, 2, 3, etc. hours).
In our laboratory, the viability of the brain slices is assessed by measuring the relative activity of released lactate dehydrogenase (LDH) using a cytotoxicity detection kit [ 24] according to the manufacturer's instructions (Roche Diagnostics GmbH, Germany), with some modifications.
To calculate the viability of the brain slices (as a percentage) after the 5 h incubation-equilibration period, the following steps are taken for each experimental setup: Background absorbance control - provides information about the background absorbance of the assay medium (aECF).
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Usually, the viability of these brain slices is maintained by bathing the slices in continuously flowing oxygenated artificial cerebrospinal fluid (aCSF) in a perfusion chamber.
Postmortem analyses were carried out to measure indicators of malignancy, tumoural metabolism and viability of the blood-brain barrier (BBB).
We examined the utility of this perfusion method to enhance the viability of the thick organotypic brain slice cultures after 2 days and 5 days in vitro (DIV).
In this line, PW imaging gives crucial information related to angiogenic events in the glioma and viability of the vessels in the brain.
Copper oxide NPs reduced the viability of primary rat brain astrocytes and enhanced ROS production [94].
Although it has been shown that MTT is appropriate to evaluate the viability of brain tissue slices and its reduction is impaired after detrimental treatment, such as OGD [5], [22], the influence of Aβ on MTT reduction in OHCs has never been tested before.
Also, the effectiveness of HBE and quercetin was validated, to counteract the deleterious effects induced by C. d. terrificus venom, on cell viability of mouse brain slices.
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