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Additionally, post-thaw viability of samples were determined after a rapid thaw and 15 min incubation period using a fluorescence membrane dye exclusion assay.
The cell viability of samples at each concentration of the drugs or chemicals was calculated by dividing the optical density of samples with that of control.
It is noteworthy that in our study, the viability of samples was verified up to 270 days after saliva collection.
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Previously recognised errors in sampling such as incorrect sample volume and mishandling by general practice or laboratory staff can lead to non-viability of samples 12 69 and were identified as an ongoing problem.
The viability of ccRCC samples compared with normal samples after 24 h treatment with increasing concentrations of 3BrPA is shown in Figure 3c.
Figure 9 Relative cell viability of control samples (no Ag-ZnO NRs) and Ag ZnO NRs.
Viability of all samples was ≥ 90%.
Cell viability of these samples were measured 15 min following exposure by TB staining.
The mean RBC recovery value after 24 hours of injection of labeled RBC was 97.5 ± 19%, demonstrating a good viability of the samples.
The control value corresponding to untreated cells was defined as 100% and the viability of treated samples was expressed as a percentage of the control.
The control value corresponding to untreated cells was taken as 100% and the viability of treated samples was expressed as a percentage of the control.
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