In all luciferase experiments, transfection efficiency and cell viability were controlled for by cotransfecting a Renilla luciferase control vector (pRLTK; Promega, Southampton, UK), and reporter firefly luciferase values were normalized to the Renilla values.
The viability of the cells was controlled by flow cytometry with propidium iodide staining and was found to exceed 95%.
The injection capillary was held in the cell for at least 20 minutes, and viability of injected cells was controlled by monitoring cytoplasmic streaming during the microinjections.
Viability of the cells, which was controlled by trypan blue dye exclusion or by quantitative fluorescence analysis of propidium iodide uptake, was > 90%.
Tumour growth and viability of the embryo were controlled daily by stereo microscopy.
Tumour growth and viability of the developing embryo were controlled daily by stereo microscopy.
Bacterial viability in the broth containers was controlled after inoculation by microscopy and cultivation.
The viability of the overall process is actually controlled by the mutual affinity between the VOCs, the solvent phase and the polymeric material.
In control experiments, cell viability is verified by the addition of a two-photon fluorescence channel to the OCM.
Cell viability is obtained by MTS assay.
